Overview of Nonionic Surfactant

Nonionic surfactants are surface-active agents that do not ionize in aqueous solutions. They are widely used in various industries, including detergents, cosmetics, and pharmaceuticals, due to their excellent solubilizing, emulsifying, and wetting properties. These surfactants are derived from alcohols, acids, or amines and are known for their versatility and compatibility with other surfactants.

Features of Nonionic Surfactant

Non-Ionic Nature: Do not form ions in water, making them less sensitive to hard water and electrolytes.

Versatility: Compatible with both anionic and cationic surfactants, allowing for broad formulation flexibility.

Solubilization: Excellent at solubilizing oils and other hydrophobic substances.

Emulsification: Effective in creating stable emulsions, which is crucial for many cosmetic and pharmaceutical formulations.

Wetting Ability: Enhances the wetting of surfaces, improving cleaning efficiency.

Low Foaming: Generally produce less foam compared to other types of surfactants, which can be advantageous in certain applications like industrial cleaning.

Temperature Stability: Maintain effectiveness over a wide range of temperatures, making them suitable for high-temperature processes.

Environmental Friendliness: Some nonionic surfactants are biodegradable, contributing to more environmentally friendly products.

(Triton X-100 Molecular Biology Nonionic Surfactant)

Specifications of Triton X-100 Molecular Biology Nonionic Surfactant

Triton X-100 Molecular Biology Grade Nonionic Surfactant is a high-purity, non-denaturing detergent extensively used in biochemical and molecular biology applications for its ability to solubilize healthy proteins while maintaining their indigenous structure and activity. This surfactant is maximized for delicate methods, making sure marginal interference with chemical responses, immunoassays, and mobile researches.

** Chemical Qualities **: The energetic ingredient is Octoxynol-9 (polyethylene glycol tert-octylphenyl ether), with the molecular formula C ₃₄ H ₆₂ O ₁₁ and a molecular weight of around 625 g/mol. It is provided as a thick, clear to light yellow fluid with a small fragrant smell. Triton X-100 is totally soluble in water, ethanol, and ethylene glycol, creating colorless options. The important micelle focus (CMC) is 0.015% (w/v) in liquid services, with a hydrophilic-lipophilic balance (HLB) of 13.5.

** Purity & High quality **: The molecular biology grade makes sure ultra-purity, with ≥ 98% energetic ingredient material. It is evaluated for reduced endotoxin degrees (

(Triton X-100 Molecular Biology Nonionic Surfactant)

Applications of Triton X-100 Molecular Biology Nonionic Surfactant

Triton X-100 is a nonionic surfactant commonly used in molecular biology for its capability to solubilize proteins, interfere with mobile membrane layers, and preserve healthy protein stability in aqueous services. Its moderate detergent buildings make it suitable for applications calling for the preservation of organic task while efficiently permeabilizing membranes or isolating mobile parts.

In cell lysis, Triton X-100 is a best reagent for breaking open cell membrane layers without denaturing proteins. It solubilizes lipid bilayers and launches cytoplasmic or membrane-bound proteins, making it important for removing enzymes, receptors, or antigens. This is particularly beneficial in studies including Western blotting, ELISA, or protein filtration, where preserving native protein conformation is important.

For healthy protein evaluation, Triton X-100 aids in avoiding healthy protein gathering during electrophoresis or chromatography. It maintains hydrophobic healthy proteins in remedy, guaranteeing exact separation and detection. In immunoassays, it is utilized to permeabilize cells for antibody penetration while reducing nonspecific binding, enhancing signal clarity in methods like immunofluorescence or circulation cytometry.

Triton X-100 also plays a role in nucleic acid isolation. By lysing cell membrane layers and suspending nucleases, it facilitates the launch of DNA or RNA while preserving their honesty. Its compatibility with chemical responses makes it appropriate for PCR barriers or restriction digest procedures.

In cell society, reduced concentrations of Triton X-100 are employed to permeabilize live or fixed cells for intracellular discoloration or transfection researches. Its mild activity ensures marginal interruption to cellular design, enabling specific visualization of subcellular frameworks.

Additionally, Triton X-100 is made use of in analysis kits and research study assays to improve reagent security and solubility. Its uniformity and dependability in solubilizing hydrophobic compounds contribute to reproducible experimental outcomes.

Safety-wise, Triton X-100 works with many biochemical barriers however calls for cautious handling because of its thick nature. Optimal focus vary by application, with excess quantities potentially hindering downstream processes.

Overall, Triton X-100’s convenience in membrane layer disturbance, healthy protein stabilization, and assay optimization solidifies its function as a foundation reagent in molecular biology workflows, from basic research to analysis applications.

Company Introduction

Welcome to Robocup, a premier global supplier of high-quality surfactants. Our extensive range includes anionic, cationic, nonionic, and amphoteric surfactants, catering to industries such as personal care, textiles, cleaning, and industrial applications. With advanced manufacturing facilities and rigorous quality control, we ensure that our products meet the highest international standards. We pride ourselves on our commitment to innovation, sustainability, and customer satisfaction. Our dedicated team provides tailored solutions to meet your specific needs. Partner with us for reliable, high-performance surfactants that drive your business forward. Explore our offerings and discover the difference today.

If you have any questions, please feel free to contact us(nanotrun@yahoo.com).

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By air, by sea, by express, as customers request.

5 FAQs of Triton X-100 Molecular Biology Nonionic Surfactant

Triton X-100 is a nonionic surfactant widely used in molecular biology for lysing cells, solubilizing membrane proteins, and permeabilizing cellular membranes without denaturing proteins. It is ideal for applications like immunofluorescence, flow cytometry, and protein extraction, where maintaining protein function and structure is critical. Its mild nature makes it suitable for disrupting lipid bilayers while preserving protein-protein interactions.

A typical working concentration ranges from 0.1% to 1%, depending on the application. For cell lysis, 0.5–1% is common, while immunofluorescence staining may require lower concentrations (0.1–0.5%). Always optimize based on experimental conditions to balance efficacy and minimal cellular disruption.

Triton X-100 is compatible with many ionic and nonionic detergents, such as SDS or CHAPS, when used in mixed micelle formulations. However, compatibility depends on the specific protocol—consult literature to avoid interference. It is not recommended for use with strong oxidizing agents or in assays where its UV absorbance (below 280 nm) might interfere.

Handle Triton X-100 with gloves and eye protection, as it can cause skin and eye irritation. Store at room temperature (15–25°C) in a tightly sealed container, protected from light. Avoid freezing, as it may solidify and require gentle warming to redissolve. Properly stored, it remains stable for years.

Compared to Tween-20, Triton X-100 is more effective at disrupting lipid-rich membranes and has a lower critical micelle concentration (CMC), enhancing its solubilizing power. Unlike NP-40, it may exhibit slightly different selectivity for membrane proteins. Triton X-100 is milder than ionic detergents like SDS, making it preferable for native protein studies. Choose based on solubility requirements and experimental goals.

(Triton X-100 Molecular Biology Nonionic Surfactant)